Guidelines for Minimal Information on Cellular Senescence Experimentation in vivo
- Metin Sokmen, MD
- 25 Ağu 2024
- 1 dakikada okunur
🔶Brain:
🔹Both proliferative glial cells and post-mitotic neurons can be positive for markers of cellular senescence.
🔹Markers like SA-β-gal are detectable even in neurons of young and healthy mice, raising questions about their suitability for assessing senescence in the brain.
🔶Skin:
🔹Markers like p21, p16Ink4a, γ-H2A.X, and TAF/TIF have been identified in skin cell types such as keratinocytes and fibroblasts.
🔹Autofluorescent signals should be reduced or signals detected using far-red or near-infrared detection when detecting proteins with low expression in skin sections.
🔶Lungs:
🔹Increased SA-β-gal activity, p21, and γ-H2A.X levels have been used to detect senescence in bronchial epithelial cells exposed to chronic inflammation.
🔶Bone:
🔹With aging, there is an increase in p16+, KI67-, BCL2+, and SASP+ cells in bone mesenchymal cells.
🔹The clearance of p21+ senescent cells, but not p16+, prevents radiation-induced bone loss.
🔶Liver:
🔹Markers such as SA-β-gal, DNA damage, TAFs, karyomegaly, and SASP factors are found in various liver cell types during aging and injury.
🔹Hepatocytes, in particular, display these senescence markers.
🔶Adipose Tissue:
🔹Numerous senescence markers such as p21, p16, SASP factors, and DNA damage are found across populations of adipose tissue cells.
🔹p16+ cells may predominate in aged adipose tissue, whereas p21 predominates in obesity.
🔶Skeletal Muscle:
🔹A distinct subset of muscle cells shows CDKN2A mRNA, TAFs, SADS, and a unique SASP, indicating senescence.
